银杏叶中银杏酸含量的测定

陈君; 陈岑; 任红; 黄海波; 乔洪翔; 王如伟

doi:10.13748/j.cnki.issn1007-7693.2018.01.016

银杏叶中银杏酸含量的测定

Determination of Ginkgolic Acids in the Ginkgo Leaf

摘要

摘要: 目的建立高效液相色谱法测定银杏叶中银杏酸含量的方法。方法样品经甲醇超声提取，采用C₁₈柱（4.6 mm×150 mm，5 μm），流动相为含0.1%三氟乙酸的乙腈（A）-0.1%三氟乙酸水（B），梯度洗脱（0~30 min，75%→90% A；30~35 min，90% A；35~36 min，90%→75% A；36~45 min，75% A），流速1 mL·min^-1，检测波长310 nm。结果样品经甲醇超声提取，快速简便制备银杏酸供试品，白果新酸浓度在0.917~45.85 μg·mL^-1内呈良好的线性关系（n=6，r²=0.999）；平均回收率为97.34%，RSD为0.57%。结论该方法灵敏、准确、简便，适合用于银杏叶中银杏酸含量的测定。

Abstract: OBJECTIVE To develop an HPLC method for the determination of ginkgolic acids content in the Ginkgo leaf. METHODS The sample was extracted by ultrasonic extraction with methanol as the solvent, the separation was performed on C₁₈ column(4.6 mm×150 mm, 5 μm), with 0.1% trifluoroacetic acid solution of acetonitrile(A)-0.1% trifluoroacetic acid aqueous solution(B) as mobile phase, and eluted with a gradient program(0-30 min, 75%A→90%A; 30-35 min, 90%A; 35-36 min, 90%→75%A; 36-45 min, 75%A), flow rate 1 mL·min^-1, and UV detection wavelength at 310 nm. RESULTS It was convenient to prepare the sample of Ginkgolic acids by ultrasonic extraction with methanol as the solvent. The linear range for the content of ginkgoneolic acid was 0.917-45.85 μg·mL^-1(r²=0.999), the average recovery was 97.34%, and RSD was 0.57%. CONCLUSION The method is sensitive, accurate and speedy, therefore suitable for the determination of Ginkgolic acids content in the Ginkgo leaf.

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