Abstract: OBJECTIVE To investigate the effect of 95% ethanol extract of Shengji Huayu formula on wound healing in diabetic mice. METHODS In vivo animal experiment was used to study the effect of Shengji Huayu formula 95% ethanol extract(low, medium and high concentration) to promote the therapeutic effect of diabetic wound; based on diabetic mice (db/db mice), gross observation of the wound area, wound healing time, HE staining (new collagen repair, granulation, epidermal migration) comprehensive analysis data to understand the situation of wound healing. RESULTS On the eleventh day of treatment, the wound area of positive drug group and 95% ethanol group was significantly smaller than the model group (P<0.05), the wound healing rate of 95% ethanol extracts of low concentration was significantly higher than that in the middle, high concentration (P<0.05). CONCLUSION The 95% ethanol extract of Shengji Huayu formula can obviously promote wound healing.