Abstract: OBJECTIVE To evaluate eye irritation potential of different shampoos and facial creams using human cornea-like epithelial tissue model BioOcular^TM ET₅₀ method in vitro. METHODS Different shampoos and facial creams were tested in eye irritation ET₅₀ test with reconstructed human cornea-like epithelial tissue model BioOcular^TM in vitro, distinguish eye irritation levels of different formulations. RESULTS Under the same condition, shampoos and facial creams showed irritation at various levels in ET₅₀ test with reconstructed cornea-like epithelial tissue model. Also different ingredients and different brand of shampoos showed different degrees of irritation. CONCLUSION It is a quick alternative way to use ET₅₀ method to evaluate the irritation of different cosmetics and ingredients with reconstructed human cornea-like epithelial tissue model BioOcular^TM in vitro.