Abstract: OBJECTIVE To investigate the purification technology of total flavonoids from the peels of Citrus Changshan-huyou Y.B. Chang by macroporous resin. METHODS Eight macroporous resins were chosen with static and dynamic adsorption and desorption experiments to optimize the purification parameters. RESULTS HPD-300 macroporous resin was found to have good adsorption and desorption effects. The optimal purification conditions were pH 4.0, sample mass concentration of 3.83 mg·mL^-1, the loading amount of 5 mL·g^-1, the loading flow rate of 2.0 BV·h-1. The sample was eluted by 2 BV of water and 4 BV of 70% ethanol respectively with the eluting flow rate of 2.0 BV·h-1. The content of total flavonoids increased to 76.21% after the purification, and the yield was 93.94%. CONCLUSION HPD-300 is an ideal resin with satisfactory enrichment for separating and purifying the total flavonoids from peels of Citrus Changshan-huyou Y.B. Chang.