| 引用本文: | 刘影,刘涛,赵晓军,李星,杨菁.地黄寡糖对二型糖尿病大鼠糖脂代谢及肝脏超微结构的影响[J].中国现代应用药学,2014,31(11):1312-1316. |
| LIU Ying,LIU Tao,ZHAO Xiaojun,LI Xing,YANG Jing.Effect of Rehmannia Glutinosa Oligosaccharide on Glucose and Blood Lipid Metabolisms and Liver Ultrastructure in Type 2 Diabetic Rats[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(11):1312-1316. |
|
| 摘要: |
| 目的 观察地黄寡糖对2型糖尿病大鼠糖脂代谢的影响及肝脏的保护作用。方法 取♂SD大鼠用高脂高糖饲料喂养8周后，腹腔注射链脲佐菌素25 mg·kg-1制备糖尿病大鼠模型，造模成功后随机分组为：糖尿病模型组、地黄寡糖高剂量组、地黄寡糖中剂量组、地黄寡糖低剂量组、二甲双胍组，每组10只；另取8只用普通大鼠饲料喂养8周后，腹腔注射等体积柠檬酸-柠檬酸钠缓冲液，作正常对照组。在第0，2，4，8周末各测定一次空腹血糖(FBG)；第8周末测定血清甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)、和空腹血清胰岛素(FINS)；实验结束处死大鼠，取肝组织检测谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)水平；HE染色后光镜观察肝脏病理变化；透射电镜观察肝脏超微结构的改变。结果 与糖尿病模型组比较，地黄寡糖中、高剂量组FBG、TG、TC、LDL-C、FINS水平均明显降低，HDL-C升高(P<0.05)；糖尿病大鼠肝组织氧化应激水平增高，地黄寡糖中、高剂量组糖尿病大鼠SOD、GSH-Px活性均显著增加，MDA水平显著下降；HE染色显示，各地黄寡糖治疗组大鼠肝细胞脂肪变性明显减少，肝细胞排列基本规则。透射电镜结果显示，地黄寡糖高剂量组大部分线粒体结构清晰内质网完整。结论 地黄寡糖可降低糖尿病大鼠血糖、血脂，减轻肝细胞脂肪变性，从而达到保护肝脏的作用，其作用机制可能与其增强肝组织抗氧化作用，减缓糖尿病对肝组织的损伤有关。 |
| 关键词: 地黄寡糖 二型糖尿病 肝脏超微结构 |
| DOI： |
| 分类号: |
| 基金项目:辽宁省科技厅项目(2013225305)；辽宁医学院校内青年科技基金(Y2012E022) |
|
| Effect of Rehmannia Glutinosa Oligosaccharide on Glucose and Blood Lipid Metabolisms and Liver Ultrastructure in Type 2 Diabetic Rats |
|
LIU Ying1, LIU Tao1, ZHAO Xiaojun1, LI Xing2, YANG Jing1
|
|
1.Liaoning Medical University, Jinzhou 121001, China;2.Jinzhou Aohong Pharmaceutical Co., Ltd., Jinzhou 121013, China
|
| Abstract: |
| OBJECTIVE To investigate the effects of rehmannia glutinosa oligosaccharide(ROS) on glucose and blood lipid metabolisms and protective effects on liver in type 2 diabetic rats. METHODS Fifty-two male SD rats were fed with high fat and sugar for 8 weeks. After that they were injected with streptozotocin(STZ) to form diabetic model rats. The modeled rats were divided into model group, low, middle, high dose of ROS groups, and metformin group. Another 8 SD rats were selected and fed with normal food as the control group, and injected with equal-volume critric acid buffer solution. On the 0, 2, 4, 8 weekend, the level of blood glucose was determined, Blood lipid levels and fast insulin (FINS) were measured. The concent of malondialdehyde (MDA) and the activity of superoxide dismutases (SOD) and glutathione peroxidase (GSH-PX) of liver tissue were determined in killed rats. Morphological change was examined by HE staining. Liver ultrastrure was determined by TEM. RESULTS Compared with the model group, the levels of FBG, TC, TG, LDL-C, FINS obviously decreased, HDL-C significantly increased, the activity of SOD and GSH-PX increased, MDA decreased significantly in the liver tissues in high, middle dose of ROX groups. HE staining showed that liver fatty degeneration was significantly reduced in ROS groups. TEM results showed that: ROS high-dose group showed mitochondrial structure clear and endoplasmic reticulum in tegrity. CONCLUSION These results suggest that ROS can reduce blood sugar, lipids levels in diabetic rats, relieve fatty degeneration of liver cells, and its mechanism may be related to antioxidation and attenuating liver injury induced by dabete. |
| Key words: rehmannia glutinosa oligosaccharide type two diabetic liver ultrastructure |
