| 引用本文: | 陆欢欢,周旭烨,张智武.I型胶原蛋白对斑马鱼模型促组织再生及抗炎作用研究[J].中国现代应用药学,2022,39(9):1182-1186. |
| LU Huanhuan,ZHOU Xuye,ZHANG Zhiwu.Study on Effect of collagen type I on Tissue regeneration and anti-inflammation in zebrafish model[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(9):1182-1186. |
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| 摘要: |
| 目的 观察I型胶原蛋白对斑马鱼模型的促组织再生及抗炎作用。方法 通过检测斑马鱼尾鳍再生面积和col1a1b基因的相对表达量，表征I型胶原蛋白促组织再生作用；通过测量斑马鱼皮肤中性粒细胞数量变化表征I型胶原蛋白的抗炎作用。结果 与模型对照组相比，I型胶原蛋白溶液处理斑马鱼尾鳍面积明显增大，中性粒细胞数量明显减少。I型胶原蛋白溶液处理后的斑马鱼col1a1b基因的相对表达量明显高于正常对照组。结论I型胶原蛋白对斑马鱼模型具有促组织再生及抗炎作用。 |
| 关键词: I型胶原蛋白 斑马鱼模型 组织再生 抗炎 |
| DOI：10.13748/j.cnki.issn1007-7693.2022.09.008 |
| 分类号:R965.1 |
| 基金项目: |
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| Study on Effect of collagen type I on Tissue regeneration and anti-inflammation in zebrafish model |
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LU Huanhuan1,2, ZHOU Xuye2, ZHANG Zhiwu2
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1.Zhejiang Chinese Medical University, Hangzhou 310053, China;2.Zhejiang Chunsum Biological Products Co., Ltd., Hangzhou 311100, China
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| Abstract: |
| OBJECTIVE To observe the effects of collagen type I on tissue regeneration and anti-inflammation in zebrafish model. METHODS The effect of collagen type I on tissue regeneration was characterized by measuring the regeneration area of zebrafish caudal fin and the relative expression of col1a1b gene. The anti-inflammatory effect of collagen type I was characterized by measuring the number of neutrophils in the skin of zebrafish. RESULTS Compared with the model control group, the caudal fin area of zebrafish treated with type I collagen solution increased significantly, while the number of neutrophils in zebrafish decreased significantly. The relative expression of col1a1b gene in zebrafish treated with type I collagen solution was significantly higher than that in normal control group. CONCLUSION Type I collagen has the effects of promoting tissue regeneration and anti-inflammation in zebrafish model. |
| Key words: collagen type I zebrafish model tissue regeneration anti-inflammatory |
