Protective Effect of Tibetan Medicine Potentilla Anserina L. on Ethanol-induced Damage in Prime Liver Cell of Mice

OBJECTIVE To study the protective effct of Tibetan medicine Potentilla anserina L. on ethanol-induced damage in mice prime liver cell. METHODS After isolation, purification and culture of primary hepatocytes, the livability of cell was evaluated by MTT; fluorescence microscopy were used to evaluated the contents of reactive oxygen species (ROS) and concentration of Ca²⁺; flow cytometry was used to detect the cell apoptosis; the expression of Bcl-2 and Bax were detected by Western bloting. RESULTS After damaged by alcohol, the cell livability decreased, the contents of Ros and concentration of Ca²⁺ increased, the expression of Bcl-2 decreased while that of Bax increased. Potentilla anserina could increase the cell livability, decreased the intracellular ROS content and Ca²⁺ concentration ameliorate the apoptosis, increase the expression of Bcl-2, and decrease the expression of Bax. CONCLUSION Tibetan medicine Potentilla Anserina L. has protective effect on ethanol-induced damage in prime liver cell of mice.