Transformation of Prunin by Recombinant α-L-rhamnosidase

OBJECTIVE To investigate the transformation of prunin by recombinant α-L-rhamnosidase. METHODS The final product was identified by HPLC method. The dynamic conversion process was analyzed by DNS method, then the preparation process was optimized. RESULTS The transformation final products were prunin and rhamnose. The optimal conditions for transformation of prunin were 60 ℃, pH 4.0, enzyme dosage 12 U·mL^-1, substrate concentration 2.0 g·L^-1. Under the optimal condition, 94% naringin could convert to prunin. High concentration of Mn²⁺ and Fe²⁺ could significantly accelerate transformation of prunin. The dynamic parameters of K_m and V_max were 1.02 μmol·mL^-1 and 0.19 μmol·mL^-1·min^-1 respectively. CONCLUSION The preparation of prunin by recombinant α-L-rhamnosidase has high conversion rate, good reproducibility, and the products were easy to separate.