Effect of Nano Chinese Yam Polysaccharide on 4 Kinds of Tumor Cells

OBJECTIVE To study anti-tumor activity of nano chinese yam polysaccharide and to explore the mechanism of its action. METHODS Cell proliferation/toxicity detection kit(CCK-8 kit) was used to determine inhibition rate to tumor cells of each drug administration group; Western blotting was used to test the effect of each drug administration group on the expression of tumor cell apoptosis related protein caspase-3, caspase-8 after 48 h. Effect of each drug administration group on the ability of tumor cell migration was tested by tumor cell scratch experiment. RESULTS CCK-8 experiment showed that compared with normal control group, high dose group of nano chinese yam polysaccharide(80 ng·mL^-1) and fluorouracil group(80 ng·mL^-1) could obviously inhibit tumor cell proliferation(P<0.05); Western blotting experiment showed that the tumor cell apoptosis protein caspase-3, caspase-8 of nano chinese yam polysaccharide group(80 ng·mL^-1) and fluorouracil group(80 ng·mL^-1) had lower band grayscale, protein expression were significantly increased compared with the normal group (P<0.05); Cell scratch experiment showed that healing rate of 4 kinds of tumor cells treated with nano chinese yam polysaccharide all had varying degrees of decline(P<0.05) after 48 h. CONCLUSION The increase of the dosage of nano chinese yam polysaccharide can promote the original tumor cell apoptosis caspase-3 and caspase-8 protease activation, increase the degradation of zymogen, inhibit the growth of tumor cells, promote tumor cell apoptosis and reduce cell scratch healing rate, nano chinese yam polysaccharide has anti-tumor activity.