Simultaneous Determination of Chlorogenic Acid, Mignonette Glycosides and 3, 5-O-dicaffeoylquinic Acid of Yinju Extract by HPLC

OBJECTIVE To establish an HPLC method for the simultaneous determination the contents of chlorogenic acid, mignonette glycosides and 3, 5-O-dicaffeoylquinic acid in Yinju extract and select the best extraction solvent. METHODS Ultimate PLUS C₁₈ column was applied(4.6 mm×250 mm, 5 μm) with acetonitrile(A) and 0.1% phosphoric acid(B) as mobile phase in gradient mode. The flow rate was 1.0 mL·min^-1 with column temperature of 40?℃. The detection wavelength was set at 348 nm. The contents of 3 components in Yinju extract extracted by different extraction solvents were compared. RESULTS The linear range of chlorogenic acid, mignonette glycosides and 3, 5-O-dicaffeoylquinic acid were 0.077-1.920, 0.082-3.288, 0.141-5.624 μg and the average recoveries were 100.8%(RSD=2.3%), 101.5% (RSD=0.7%), 97.6%(RSD=0.6%). Ethanol was better than water to extract Yinju extract and 40% ethanol was the best extraction solvent. CONCLUSION This method is simple, accurate and capable for the optimization of extract process and quality control of Yinju extract.