Determination of Rosmarinic Acid and Oridonin in Rabdosia Japonica (Burm.f.) Hara by HPLC

OBJECTIVE To develop a reversed phase high performance liquid chromatography method for simultaneous determination of rosmarinic acid and oridonin in Rabdosia japonica (Burm.f.) Hara. METHODS The separation of rosmarinic acid and oridonin were performed on a Kromasil C₁₈ analytical column(250 mm×4.6 mm, 5 μm) with methanol(A)-0.1% phosphoric acid(B) as mobile phase in a gradient elution program at a flow rate of 1 mL·min^-1. The column temperature was maintained at 30 ℃. The detection wavelength was 239 nm. RESULTS Rosmarinic acid and oridonin had a good linearity ranging in 9.53-4 765 ng and 13.66-6 830 ng, respectively. The average recoveries of were 103.3% and 95.8%, RSDs were 1.4% and 1.9%, respectively. CONCLUSION The developed method is accurate with high simplify, stability, and reliability which is helpful to the quality control of Rabdosia japonica (Burm.f.) Hara.