Optimization of the Extracting Process of Total Organic Acid from Cassia Twig by Orthogonal Experiment with Ethanol Reflux

OBJECTIVE To study the optimum extraction technology of total organic acids in Cassia Twig. METHODS Using total organic acid extraction rate as index to investigate the effection of the extraction results by ethanol concentration, dosage of ethanol, extraction times with orthogonal design test. RESULTS Optimum extraction process were as follows: ethanol concentration of 90%, 8 times solvent dosage, extraction for 3 times. CONCLUSION The process proceeds higher extraction yield, stability, repeatability is good, suitable for the extraction of total organic acids.