Determination of Three Triterpenoid Acid in Ganoderma and Its Characteristic Chromatograms

OBJECTIVE To establish determination method for triterpenoid content in Ganoderma and study its of characteristic chromatograms. METHODS HPLC was applied with the chromatographic condition as follows: the chromatographic column was Agilent Zorbax SB-C₁₈(250 mm×4.6 mm, 5 μm) at 30 ℃; mobile phase was acetonitrile-0.1% glacial acetic acid (30∶70); flow rate was 1.0 mL·min^-1, the detection wavelength was 254 nm. Using the similarity evaluation system for fingerprint similarity calculation. RESULTS Ganoderma triterpene component was high, Ganoderma fingerprint similarity was above 0.93. CONCLUSION Ganoderma triterpene component content in Ganoderma is high, the characteristic chromatograms method is stable, good reproducibility, and can be used for quality control of Ganoderma.