Study of the Activity Determination of Cerebroprotein Hydrolysate on Nerve Cell Damage

OBJECTIVE To evaluation the restoration of PC12 cell by cerebroprotein hydrolysate and provide criterion for the biological activity detection method of cerebroprotein hydrolysate. METHODS Cerebrolysin, the representative cerebroprotein hydrolysate imported, was adopted as repair agent in this study. PC12 cell was adopted as the indicator of nerve cell. Effects of concentration of cell inoculations, H₂O₂ and cerebroprotein hydrolysate on PC12 restoration were studied respectively. The protection of cerebroprotein hydrolysate on PC12 model cell was determined by MTT colorimetry which colorized the sample group, control group and damage group. Restoration rates were calculated by OD values. Biological activities of domestic products were evaluated by the method also. RESULTS The PC12 cell damage model could be established by the condition of 8×10⁴-12×10⁴ per·mL^-1, 0.5 mmol·L^-1 H₂O₂ and 60 μg·L^-1 cerebroprotein hydrolysate (calculated by nitrogen content). Contrast detections were carried out using this method. CONCLUSION Domestic cerebroprotein hydrolysate of 8 batch samples and cerebrolysin were proved to be effective on the restoration of PC12 cell damaged by H₂O₂.