Study on Improved Quality Standard for Xiaobai Granules

OBJECTIVE To establish and improve the quality standard of Xiaobai granules. Methods TLC was used to identify Astragali Radix, Rehmanniae Radix, Polygoni Multiflori Radix, and Psoralen in Xiaobai granules. Astragloside Ⅳ in preparation was determination by HPLC-ELSD on Lichrospher-C₁₈(4.6 mm×250 mm, 5 μm) chromatographic column, the mobile phase was acetonitrile-water(32∶68) at a flow rate of 1.0 mL·min^-1, and column temperature was 25 ℃. RESULTS TLC spots were clear and well-separated without negative interference. The calibration curves of astragaloside Ⅳ demonstrated linear relationship in the range of 0.311 5-2.803 9 μg(r=0.999 9), average recovery rate was 99.4%(RSD=2.67%). CONCLUSION the method established is simple, accurate and reproducible, which can be used for the quality control of Xiaobai granules.