Effect of Puerarin on Proliferation Inhibition and Apoptosis of Human Osteosarcoma Cell Lines MG63

OBJECTIVE To investigate the effect and mechanisms of puerarin on the human osteosarcoma cell lines MG63. METHODS MTT method was used to evaluate the proliferation, and the percentage of apoptotic cells were determined by flow cytometric analysis. The mRNA level of PI3K, AKT, Bax, Bcl-2, p53 and p21 were detected by RT-PCR. The expression of PI3K, AKT, Bax, Bcl-2, p53 and p21 were exanimed by Western blotting. RESULTS The results showed that puerarin treatment could induce the proliferation inhibition and apoptosis of MG63 cell in a concentration-dependent manner with the up-regulating the expression of Bax and down-regulating the expression of PI3K, AKT, Bcl-2, p53 and p21 in MG63 cell. CONCLUSION Puerarin can induce apoptosis and growth inhibition of human osteosarcoma cell lines MG63 and the mechanism is associated with suppressing PI3K/AKT/p53 signal pathway.