Study on Simultaneous Determination of Three Main Marker Components in Xuanfeizhike Mixture by HPLC

OBJECTIVE To establish an HPLC method for simultaneous determination of puerarin, daidzein and glycyrrhizic acid in Xuanfeizhike mixture. METHODS Isolation of sample was achieved on an Agilent Zorbax SB-C₁₈(4.6 mm×250 mm, 5 μm), under the 254 nm detection wavelength, gradient eluted by acetonitrile-0.1% formic acid, and the column temperature was maintained at 30 ℃. RESULTS The linearity ranges of puerarin, daidzein and glycyrrhizic acid were 99.14-991.4, 22.50-225.0, 22.60-226.0 μg·mL^-1, respectively(r≥0.999 8). The average recoveries(n=9) of them were 99.2%, 98.9%, 99.3%. CONCLUSION The method is simple, accurate and has a good repeatability. The study provides scientific foundation for quality evaluation and quality control standards for Xuanfeizhike mixture.