Rosmarinic Acid Inhibiting Metastasis of Cervical Cancer by Reducing RhoA Expression

OBJECTIVE To investigate the inhibiting effect of rosmarinic acid on metastasis of cervical cancer and the possible mechanism. METHODS Cervical cancer cells were treated by rosmarinic acid with concentration of 1, 5, 10 μmol·L^-1. Transwell chamber was used to examine the metastasis of the cultured cells; semi-quantitative RT-PCR method was used to detect the expression of RhoA mRNA and immunofluorescence technique was used to detect the expression of RhoA protein. RESULTS The metastasis of cervical cancer was inhbited by rosmarinic acid with the concentration of 5, 10 μmol·L^-1 (P<0.01), and the expression of RhoA decreased significantly in the two groups(P<0.05). CONCLUSION Rosmarinic acid can inhibit the metastasis of cervical cancer, and the mechanism may be related to reducing the expression of RhoA in cells.