Simultaneous Determination of Notoginsenoside R₁, Ginsenoside Rg₁ and Ginsenoside Rb₁ in Dieda Pills by HPLC-ELSD

OBJECTIVE To establish a quantitative method using HPLC-ELSD to simultaneously determine three components(notoginsenoside R₁, ginsenoside Rg₁, ginsenoside Rb₁) in Dieda pills. METHODS A Phenomenex Kinetex C₁₈ column(100 mm×4.6 mm, 2.6 μm) was used to perform the determination, which was maintained at 30 ℃ throughout the analysis. Moble phase was composed of acetonitrile and water with flow rate at 0.5 mL·min^-1 under gradient elution. The evaporation light-scattering detector was used with its drift tube temperature at 110 ℃ and the gas flow rate: 3.0 L·min^-1. RESULTS Notoginsenoside R₁, ginsenoside Rg₁, ginsenoside Rb₁ shown good linearity within 0.158-3.16 μg(r=0.999 8), 0.407-8.14 μg(r=0.999 5), 0.446-8.92 μg(r=0.999 9), and their average recoveries were 97.55%(RSD=1.04%), 98.09%(RSD= 1.03%), 97.34%(RSD=0.81%), respectively. CONCLUSION The method is simple, rapid and accurate. It show this method could be used for the quality control of Dieda Pills.