Qualitative and Quantitative Determination of 3 Kinds of β Agonist Molecules by Surface Enhanced Raman Spectrometry

OBJECTIVE To study and establish the method for determination of β agonists in human urine by surface enhanced Raman spectrometry, taking clorprenaline hydrochloride, bambuterol hydrochloride and clorprenaline hydrochloride as exemplars. METHORDS The peaks at 800-850, 1 050 and 1 450 cm^-1 were identified as β agonist characteristics, while the assay was carried out taking clorprenaline hydrochloride at the peak 800-850 cm^-1. RESULTS The calibration curve of dorprenaline hydrochloride was linear in the range of 1-9 mg·L^-1, with the correlative coefficient R²=0.997 2. The limit of detection was 0.1 mg·L^-1. CONCLUSION The method is proved to be rapid, simple, non-invasive and authentic.