Determination of Residual Organic Solvents in Bilberry Extract by GC

OBJECTIVE To establish a method of headspace capillary GC for determination of residual organic solvents including methanol, ethanol and acetone in bilberry extract. METHODS The residual organic solvents were separated on HP-INNOWAX capillary column(30 m×0.320 mm, 0.50 μm). FID was used as detector with a temperature of 250 ℃, and the inlet temperature was 220 ℃. The carrier gas was nitrogen, and the flow rate was 2.0 mL·min^-1. The programmed column temperature was set as follows: maintained at 40 ℃ for 6 min and raised to 220 ℃ at the rate of 25 ℃·min^-1, followed by holding for 5 min. RESULTS the linear range of methanol, ethanol and acetone was 1.035-10.35, 10.018-100.18, 0.259-2.59 μg·mL^-1, with the average recovery of 97.95%, 98.24% and 98.10%, respectively. CONCLUSION the detection method is sensitive, accurate and reliable, and can be used in the quality control of bilberry extract.