Preparation and Molecular Recognition Capability of Chromatographic Column of Valsartan- molecularly Imprinted Polymer

OBJECTIVE To explore the molecule recognition capability of valsartan-molecularly imprinted polymer(Val-MIP) by HPLC. METHODS The chromatographic column of Val-MIP was synthesized by using valsartan as the template. Chromatographic performance of valsartan was investigated in different mobile phases. The Val-MIP was investigated for the recognition of valsartan and other compounds in the same mobile phase. RESULTS The Val-MIP exhibited high affinity for valsartan in the mobile phase of methanol and had good selectivity for valsartan and chlorogenic acid. The K’ value reduced as the content of polar solvent increased in the mobile phase. The Val-MIP had no selectivity in the mobile phase of methanol-acetic acid(7∶3). CONCLUSION The Val-MIP has good selectivity for valsartan and chlorogenic acid acid. The Val-MIP can be used for extracting analogs of valsartan from herbs.