Determination of Chlorogenic Acid, Mangiferin and Hesperidin in Yinhua Mangguo Tablets by HPLC

OBJECTIVE To establish an HPLC method for determination of chlorogenic acid, mangiferin and hesperidin in Yinhua Mangguo tablets. METHODS Waters SunFire^TM C₁₈(4.6 mm×150 mm, 5 μm) column was used. The mobile phase was methanol-water containing 0.2% phosphoric acid with gradient elution mode at the flow rate of 1.0 mL·min^-1; the detection wavelength was 327 nm(0-10 min) for chlorogenic acid, 258 nm(10-20 min) for mangiferin, 283 nm(20-40 min) for hesperidin. RESULTS Chlorogenic acid had a good linearity in the range of 0.140-2.516 μg, the average recovery was 99.4% with RSD of 1.2% (n=9). mangiferin had a good linearity in the range of 0.042-0.761 μg, the average recovery was 99.9% with RSD of 1.4% (n=9). hesperidin had a good linearity in the range of 0.076-1.361 μg, the average recovery was 99.9% with RSD of 0.7%(n=9). CONCLUSION The method is simple, accurate and reproducible. It can be used to determine chlorogenic acid, mangiferin and hesperidin in Yinhua Mangguo tablets.