Determination of Illegal Added Unknown Sildenafil Substances in Health Care Products by UPLC-Q-TOF-MS

OBJECTIVE To establish a UPLC/Q-TOF-MS method for the detection of illegal added unknown sildenafil substances in health care products. METHODS ZORBAX SB C₁₈ chromatographic column(4.6 mm×150 mm, 5 μm) was used. Mobile phase was acetonitrile(A)-0.1% formic acid solution(B) with the gradient elution. TOF and target MS/MS scanning mode was adopted. RESULTS The unknown sildenafil compound was found in samples. The fraction which analyzed by MSC and Chemispider was C₂₄H₃₄N₆O₄S₂. CONCLUSION This method can be used for rapid detection of unknown sildenafil substance which is illegally added in health care products.