Simultaneous Determination of Ferulic Acid, Rutin, Rhein, Emodin and Chrysophanol in Huangshiganmao Tablets by HPLC

OBJECTIVE To establish an HPLC method for the simultaneous determination of ferulic acid, rutin, rhein, emodin and chrysophanol in Huangshiganmao tablets. METHODS A Welch Topsil-C₁₈(4.6 mm×250 mm, 4 μm)column was used with the mobile phase of methanol-0.1% phosphate solution(78∶22) at a flow rate of 1.0 mL·min^-1, the column temperature of 30 ℃ and the detection wavelength was 280 nm. RESULTS The calibration curves of ferulic acid, rutin, rhein, emodin and chrysophanol were linear over the ranges of 0.020 82-0.416 3 μg·mL^-1(r=0.999 7), 0.011 32-0.278 3 μg·mL^-1(r=0.999 3), 0.017 22-0.344 3 μg·mL^-1(r=0.999 5), 0.015 79-0.315 8 μg·mL^-1(r=0.999 6) and 0.051 34-1.027 μg·mL^-1(r=0.999 9) respectively, and the average recoveries were 97.4% (RSD=1.1%), 95.0% (RSD=0.88%), 97.5% (RSD=1.3%), 97.4% (RSD=1.4%) and 96.3%(RSD=0.87%) respectively. CONCLUSION This method was simple, accurate and can be used for quantitative analysis method of Huangshiganmao tablets.