Content Determination of Antioxidants PEPQ in Infusion of 3-layer Co-extrusion Bags by HPLC

OBJECTIVE To develop a method for content determination of PEPQ infusion of 3-layer co-extrusion bags by HPLC. METHODS The analysis was performed on Dikma Diamonsil C₁₈(250 mm×4.6 mm, 5 μm) column, using acetonitrile-tetrahydrofuran(80∶20), Column temperature 30 ℃ and detection wavelength 270 nm. RESULTS The method has a linear range between 0-200.0 μg·mL^-1(r=0.999), The average recovery of antioxidants PEPQ was 92.3%(RSD=1.97%). CONCLUSION This method is accurate, sensitive, simple, it can be used for content determination of antioxidants PEPQ in infusion of 3-layer co-extrusion bags.