Determination of the Content and Content Uniformity of Atropine Sulfate Tablets by HPLC

OBJECTIVE To establish an HPLC method for the determination of the content and content uniformity of atropine sulfate tablets. METHODS SHISEIDO CAPCELL C₁₈ chromatographic column(250 mm×4.6 mm, 5 μm) was adopted. The mobile phase was 0.01 mol·L^-1 potassium dihydrogen phosphate solution(including 0.15% triethylamine, adjusted to pH 6.0 by phosphoric acid)-methanol(75∶25) at the flow rate of 1.0 mL?min^-1. The detection wavelength was 210 nm, and the column temperature was 35 ℃. The injection volume was 20 μL. RESULTS Good linear correlation was found in the range of 5.878 8-58.788 μg·mL^-1, and the linear equation was Y=24.015 8X+0.095 9, r=1.000 0. The average recovery was 100.2%(n=9). CONCLUSION This method is simple, rapid and efficient for the quality control of atropine sulfate tablets.