Effect of Extracts of Sibraea Angustata (Rchd.) Hand-Mazz. on the Enzyme Activity and mRNA Expression of CYP2E1 in Mice with Isoniazid-induced Liver Injury

OBJECTIVE To explore the effect of the extracts of Sibraea angustata (ESA) on gene expression of CYP2E1 in mice with liver injury induced by isoniazid(INH). METHODS Sixty mice were randomly divided into normal control group, model control group, positive control group (Silybin capsules) and three doses(1.0, 1.5, 2.0 g·kg^-1) groups of ESA. After intragastric administrated with corresponding drugs for 10 days, the biochemical indicators in surum(AST, ALT) and liver (MDA, SOD, GSH-PX), histological patterns, the activity and mRNA epression of CYP2E1 in mice were measured and compared. RESULTS Compared with model control group, the activity of related biochemical indexs ameliorated significantiy(P<0.05 or 0.001), which were confirmed by histopathological analysis. The enzyme activity and mRNA expression of CYP2E1 decreased significantly in ESA-treated groups (P<0.05). CONCLUSION ESA may exerts its hepatoprotective activity against INH-induced liver injury by inhibiting the activity and expression of CYP2E1 andproducyion free radical.