Study on the Quality Investigation and Fingerprint of Aurantii Fructus

OBJECTIVE To investigate the quality of the 16 batches of commercial Aurantii Fructus by establishment of HPLC fingerprinting. METHODS HPLC was applied with the chromatographic condition as follows: the chromatographic column was Agilent Zorbax SB-C₁₈ (250 mm×4.6 mm, 5 μm) at 30 ℃; mobile phase was acetonitrile-water(20∶80)(adjusted to pH 3 with phosphoric acid); flow rate was 1.0 mL?min^-1, the detection wavelength was 283 nm. Used the similarity evaluation system for fingerprint similarity calculation. RESULTS Sixteen batches of commercial Aurantii Fructus were reaching pharmacopoeia standards, there were a little differences between two processing methods. Fingerprint similarity was above 0.98. CONCLUSION The method of fingerprint is stable, repeatable and it is suitable for the quality control of Aurantii Fructus.