Simultaneous Determination of Epigoitrin, Chlorogenic Acid and Liquiritin in Child Kechuanling Granules by Wavelength Conversion HPLC

OBJECTIVE To determine the content of epigoitrin, chlorogenic acid and liquiritin in child kechuanling granules by wavelength conversion HPLC. METHODS The sample was extracted by 70% methanol and separated on a Inertsil ODS-SP C₁₈(4.6 mm×250 mm, 5 μm). The mobile phase were 0.1% phosphoric acid and acetonitrile, gradient elution. The flow rate was 1.0 mL·min^-1 while the ultraviolet detector was operated at 245 nm(0-13 min) and 217 nm(13-52 min). RESULTS The linear range of epigoitrin, chlorogenic acid and liquiritin was respectively in 0.700-14.000 μg·mL^-1, 4.780-95.600 μg·mL^-1, 1.050-21.008 μg·mL^-1 while the average recovery was 98.8%, 102.8%, 97.7% with the RSD 1.2%, 1.9%, 1.4%(n=9). CONCLUSION This method is simple, rapid and with good repeatability, so it could be used in quality control of child kechuanling granules.