Determination of Scopoletin in Lycii Fructus and Wuzi Yanzong Pill by HPLC-Fluorescence Detection

OBJECTIVE To develop an HPLC-fluorescence detection(HPLC-FLD) method for the determination of scopoletin in Lycii Fructus and Wuzi Yanzong pill. METHODS The chromatographic separation was performed on an Agilent Extend-C₁₈ column (4.6 mm×250 mm, 5 μm) with the mobile phase of acetonitrile-0.1% phosphoric acid (18∶82). The flow rate was 1.0 mL?min^-1at 30 ℃, and the detection was performed at wavelengths of 343 nm and 458 nm for excitation and emission, respectively. RESULTS The calibration curve was linear in the range of 41.63-2 082 pg (r=1), and the regression equation was Y=44 095X-44 660. The average recovery was 101.1% in Lycii Fructus, and 102.6% in Wuzi Yanzong Pill with the RSD of 2.2% and 1.1%, respectively. According to the assay result, the contents of scopoletin in Lycii Fructus collected from Ningxia province were higher than those from other origin. CONCLUSION The method is rapid, simple, sensitive and shows good specificity and reproducibility. It could be used to evaluate the genuineness of Lycii Fructus and control the quality of Lycii Fructus and Wuzi Yanzong pill.