Determination of Three Effective Components in Yuyejinhua Qingre Tablets by HPLC

OBJECTIVE To simultaneously determine geniposide, andrographolide and dehydroandrographolide in Yuyejinhua Qingre tablets by HPLC. METHODS Agilent ZORBAX SB-C₁₈ (4.6 mm×150 mm, 5 μm ) column was used. The mobile phase was methanol-water containing 0.2% phosphoric acid with gradient elution mode at the flow rate of 1.0 mL?min^-1; the detection wavelength was 238 nm(0-10 min) for geniposide, 225 nm(10-22 min) for andrographolide and 254 nm(22-35 min) for dehydroandrographolide. RESULTS Geniposide had a good linearity in the range of 0.166-2.981 μg, the average recovery was 99.4% with RSD of 1.2%. Andrographolide had a good linearity in the range of 0.146-2.621 μg, the average recovery was 99.2% with RSD of 1.3%. Dehydroandrographolide had a good linearity in the range of 0.222-3.992 μg, the average recovery was 100.1% with RSD of 1.4%. CONCLUSION The method is simple, accurate and reproducible, and can be used for quality control of Yuyejinhua Qingre tablets.