Optimum Extracting Procedure of Fanggan Granules by Orthogonal Design

OBJECTIVE To establish the optimum extracting procedure of Fanggan granules. METHODS A multi index comprehensive evaluation method by orthogonal test and HPLC was used for the determination of the extracting procedure of Fanggan granules, with four active ingredients including (R,S)-epigoitrin, praeruptorin A, amygdalin and liquiritin as the content index. RESULTS The optimum extraction process of Fanggan granules was determined as follows: herbs were extracted 3 times with 8 times of water; 1.5 h each time; ethanol concentration was 60%. CONCLUSION The optimum procedure is stable and reliable for the extraction of Fanggan granules.