Rapid Content Petermination of Berberine Hydrochloride in Berberis Heteropoda Skin by Near Infrared Diffuse Reflection Optical Spectra Combined with Partial Least Squares

OBJECTIVE To analysis berberine hydrochloride in Berberis heteropoda skin combined near infrared diffuse reflection optical spectra with partial least squares(NIR-PLSR). METHODS Establish a method of NIR diffuse reflectance optic-fiber spectroscopy combine with PLSR to analysis berberine hydrochloride in Berberis heteropoda skin. RESULTS The root-mean-square error of cross-validation(RMSECV) of the berberine hydrochloride in Berberis heteropoda skin was 0.810 2, the correlation coefficients was 0.987 1, the average recycle rate was 101.4%. Statistical test was used to analysis the dates and it showed that the difference between NIR prediction values and the values determinated by standard methods had no statistical significance. CONCLUSION It indicates that NIR diffuse reflectance optic-fiber spectroscopy combined with PLSR method could be used in rapid determination of the content of berberine hydrochloride in Berberis heteropoda Schrenk.