Online Monitor Multi-component Content of Danhong Injection in the Alcohol Precipitation Process Using Near-infrared Spectroscopy

OBJECTIVE To measure the changeable contents of multi-indicators in Danhong injection alcohol precipitation process, including danshensu, protocatechualdehyde, rosmarinic acid, salvianolic acid B and ethanol concentration by online near-infrared(NIR) spectroscopy analysis technique. METHODS HPLC and gas chromatography acquired the data of former four index components and ethanol concentration, respectively. Partial least squares regression (PLSR) was introduced to establish the multi-component models for online monitoring alcohol precipitation process of Danhong injection. RESULTS The residual predictive deviation(RPD) values of danshensu, protocatechualdehyde, rosmarinic acid, salvianolic acid B and ethanol concentration models were 5.72%, 7.46%, 8.44%, 4.41% and 5.50%, respectively; the values of correlation coefficient(R2) were 0.969 5, 0.982 0, 0.985 9, 0.948 4 and 0.967 0, respectively; the relative standart error(RSEP) values of validation sets were 5.35%, 7.59%, 6.42%, 4.51% and 2.30%, respectively. CONCLUSION NIR can be used as an online analytical method in Danhong injection alcohol precipitation process.