Determination of Mussaendoside G in Mussaenda Pubescens in Different Areas of Guangxi Province by HPLC

OBJECTIVE To establish an HPLC method for the simultaneous determination of mussaendoside G in Mussaenda pubescens in different areas of Guangxi province. METHODS Mussaendoside G was separated on Techmate C₁₈ (4.6 mm×250 mm, 5 μm) column and detected at 265 nm. The mobile phase was acetonitrile-water(38∶62). The flow rate was 1.0 mL·min^-1. RESULTS Mussaendoside G was 1inear within the range of 0.06-0.60 mg·mL^-1(r=0.999 9). The average recovery was 99.34%(RSD=2.32%). The contents of mussaendoside G in different areas were different. CONCLUSION The method is simple, rapid, and it can be used for the quality control of Mussaenda pubescens．