Chemical Constituents of the Fruits of Cassia Agnes Brenan

OBJECTIVE To study the chemical constituents of the fruits of Cassia agnes Brenan. METHODS Compounds were separated and purified by silica and Sephadex LH-20 column chromatography. Their structures were elucidated by physicochemical properties and spectral analysis. RESULTS Fifteen compounds were isolated from the ethyl acetate extraction of the seeds of Cassia agnes Brenan, and their structures were identified as (S)-5-(3,4- dihydroxyphenyl)-γ-valerolactone(1), glyceryl linoleate(2), β-sitosterol(3), aloe-emodin(4), rheinv(5), (-)-epiafzelechin(6), catechin(7), epizfzelechin-(4β→8)-epizfzelechin(8), sakuraneti(9), quercitrin(10), diosmetin(11), sitoindoside I(12), 5α,8α- epidioxy-(22E,24R)- ergosta-6,22-dien-3β-ol(13), vanillic acid(14), and daucosterol(15) through physicochemical properties and spectral analysis. CONCLUSION All the compounds were isolated from Cassia agnes for the first time except for 3, 6, 12 and 15.