Separation of Alvimopan and Its Enantiomers by HPLC Using β-cyclodextrin as Chiral Mobile Phase Additive

OBJECTIVE To separate the enantiomers of alvimopan by HPLC which was developed using chiral mobile phase. METHODS Separation was performed on a C₁₈ column(4.6 mm×250 mm, 5 μm). The mobile phase was methanol- water(concentration of 0.5% glacial acetic acid, 0.5% triethylamine and 1%β-cyclodextrin)(20∶80). The detection wavelength was set at 275 nm and the temperature was 40 ℃. RESULTS With this method, alvimopan and its enantiomers were well separated, the resolution met the requirement. CONCLUSION The method is simple, specific, sensitive, repeatable accurate and inexpensive.