Determination of Sugars in Red Ginseng, Ophiopogon Japonicus and Its Preparation by HPLC-ELSD

OBJECTIVE To establish a method of simultaneous determining for fructose, glucose, sucrose and maltose in red ginseng, Ophiopogon japonicus and Shengmai injection. METHODS Alltech Prevail Carbohydrate ES(4.6 mm×250 mm, 5 μm) was adopted with acetonitrile-water(72∶28) as molile phase. The flow rate was 1.0 mL·min^-1. The column temparature was maintained at 25 ℃. ELSD was was adopted with the drift temperature of 40 ℃. And Nitrogen pressure was 3.5 bar. RESULTS The linear range of fructose, glucose, sucrose and maltose were 1.01-20.20 mg, 0.48-9.66 mg, 0.95-19.08 mg and 0.96-19.28 μg, respectively. The corresponding average recovery rates were 95%-105%. CONCLUSION The method was simple, accurate and reproducible and can be used for the quality control of Red ginseng, Ophiopogon japonicus and its preparation of Shengmai Injection.