Determination of Matrine, Imperatorin and Osthole in Fubishu Vaginal Effervescent Tablets

OBJECTIVE To determine matrine, imperatorin and osthole in Fubishu Vaginal effervescent tablets by HPLC. METHODS The Kromasil 100-5 C₁₈(250 mm×4.6 mm, 5 μm)column with temperature at 30 ℃ was used to separate matrine. The mobile phase was acetonitrile-0.1% phosphoric acid solution(adjust the pH to 7.6 with triethylamine)(17∶83), the detection wavelength was 220 nm, the flow rate was 1.0 mL·min^-1. The Agela Innoval C₁₈(250 mm×4.6 mm, 5 μm)column with temperature at 30 ℃ was used to separate imperatorin and osthole. The mobile phase was acetonitrile-water(65∶35), the detection wavelength was 248 nm, the flow rate was 1.0 mL·min^-1. RESULTS A good linear relationships were obtained in the following range, matrine 156.0-3 120.0 ng, imperatorin 10.26-256.50 ng and osthole 36.98-924.50 ng(r=1.000 0). The average recoveries were 97.2%, 102.8% and 99.5%, respectively, and RSDs were 1.8%, 0.7% and 0.3%. CONCLUSION The method is simple, accurate, reproducible, and it can be applied to control the quality of the Fubishu Vaginal effervescent tablets.