Dissolution Determination of Acetaminophen and Moroxydine Hydrochloride in Ganmaoqing Capsule by HPLC

OBJECTIVE To establish the method of dissolution determination of acetaminophen and moroxydine hydrochloride in Ganmaoqing capsule. METHODS Paddle apparatus was used with 0.1 mol·L^-1 hydrochloric acid solution as the dissolution medium at a speed of 50 r·min^-1 and dissolution time limit was 30 min. The analysis was carried out on a ZORBAX SB-C₁₈(150 mm×4.6 mm, 5 μm) column, the mobile phase was composed of acetonitrile-0.02 mol·L^-1 sodium heptanesulfonate(15∶85) at the flow rate of 1.0 mL·min^-1 and the detection wavelength was 237 nm. RESULTS Acetamino- phen showed good linearity in the range of 4.588×10^-2-0.458 8 μg(r=0.999 8), the average recovery was 98.1% with RSD of 1.06%(n=9); moroxydine hydrochloride showed good linearity at the range of 4.654×10^-2-0.465 4 μg(r=0.999 6), the average recovery was 97.9% with RSD of 1.09%(n=9). CONCLUTION The method is convenient, accurate and practicable. It can be applied to determine the dissolution of the preparations.