Analysis of Carbapenemase Genotypes in Enterobacteriaceae Species with Reduced Susceptibility to Carbapenems

OBJECTIVE To investigate the drug resistance and carbapenemase genetypes of the Enterobacteriaceae with reduced susceptibility to carbapenems. METHODS Carbapenemases-producing phenotype of 36 strains was analyzed by modified Hodge test, PCR amplification was used to analyze carbapenemase genotypes. RESULTS The susceptibility test showed that the 36 clinical isolates were multi-resistant strains, resistant to third-generation and forth-generation of cephalosporin, carbapenems, fluoroquinolones and β-lactamases inhibitor compounds. Modified Hodge test showed that 16 of all strains produced carbapenemase. PCR amplification indicated that 10 strains carried the carbapenemase genetype of bla_KPC. Genes of bla_IMP, bla_VIM, bla_GIM, bla_SIM, bla_NDM-1 were not detected in all strains. CONCLUSION The mainly carbapenem-resistant mechanism of Enterobacteriaceae is producing KPC enzyme in Taizhou Hospital. KPC genes are the most popular carbapenemnases coding genes.