HPLC Characteristic Chromatograms of Formula Granule of Artemisiae Scopariae Herba

OBJECTIVE To establish the HPLC characteristic chromatogram of Formula Granule of Artemisiae Scopariae Herba. METHODS The chromatograms were obtained using Megres5-C₁₈ (250 mm×4.6 mm, 5 μm) with mobile phase consisted of acetonitrile and 0.1% phosphoric acid solution in gradient elution at flow rate of 1.0 mL·min^-1, detection wavelength of 327 nm, column temperature of 30 ℃ and sample volume of 10 μL. RESULTS The HPLC characteristic chromatogram of Formula of Artemisiae Scopariae Herba consisted of 8 common peaks. The similarity of 11 batches of samples was within 0.963-0.998. CONCLUSION The HPLC method is proved to be accurate, stable and reproducible, and it can provide reference for the identification and quality control of Formula Granule of Artemisiae Scopariae Herba.