Simultaneous Determination of Forsythoside B, Acteoside and Isoacteoside in Luohua Zizhu Tablet by HPLC

OBJECTIVE To develop an HPLC method for simultaneous determining the contents of forsythoside B, acteoside and isoacteoside in Luohua Zizhu tablet. METHODS Using 50% ethanol ultrasonic extract the sample, the chromatographic analysis was carried out on Agilent ZORBAX SB-C₁₈ column(4.6 mm×250 mm, 5 μm). The mobile phase was acetonitrile and 0.4% phosphoric acid(18.2∶81.8). The flow rate was maintained at 0.8 mL·min^-1. The detection wave length was set at 327 nm and the column temperature was controlled at 30 ℃. The sample injection volume was 10 μl. RESULTS The calibration curves were linear over the ranges of 0.936-4.680, 1.852-9.260, 2.140-10.700 μg (r=0.999 1, 0.999 9, 0.999 9) for the above compounds, respectively. The average coveries were 100.07%, 99.98%, 97.78%. CONCLUSION For this simple, rapid and accurate method is suitable for quality control and determination of products of Callicarpa nudiflora Hook. et Arn.