Study on the Drug Combination of Cisplatin and Baicalin in LKB-1 Differential Expression Cell Lines

Baicalin, one of the major flavoniods in Scutellaria baicalensis, is a potent activity in anti-inflammatory, anti-tumor and promoting metabolism. AMP-activated protein kinase(AMPK) plays an important role in energy metabolism and regulated mainly by two upstream kinases, LKB-1 or CaMKK. Recent studies show that CaMKKβ is involved in AMPK by baicalin in LKB-1 deficient cell lines. In this study, isobologram and Chou-Talalay was respectively applied to qualitative and quantitative assessment of the interaction of cisplatin and baicalin in LKB-1 deficient or sufficient cell lines. In the apoptosis results, it was found that compared with LKB-1 sufficient tumor cell lines, baicalin could enhance the rate of apoptosis induced by cisplatin in deficient cells. On the contrary, baicalin protected against the development of apoptosis in LO2 cells, a LKB-1 sufficient liver cell lines.