Study on Screening of High Quality Sarcandra Glabra in Different Provenance

OBJECTIVE To screen high quality Sarcandra glabra by determine biomasses and active components of astilbin, rosmarinic acid and isofraxidin in leaves and stems from 46 provenances. METHODS Fourty-six samples of different geographic provenance Sarcandra glabra were collected from Fujian Sanming cultivation base of the nation’s largest planting scale, random sampling of 20 strains of above ground part, separate leaves and stems, dried, weighed, calculated as per 10 of leaves, stems weight as biomasses. Determined content by HPLC. Chromatographic column: Ultimate C₁₈(250 mm×4.6 mm, 5 μm); mobile phase: A: acetonitrile-0.2% phosphoric acid(18∶82), B: acetonitrile-0.2% phosphoric acid (30∶70), 0~30 min, A→B gradient elution; flow rate: 1 mL·min^-1; column temperature: room temperature; detection wavelength: stems(determination of rosmarinic acid, isofraxidin) was 344 nm, and leaves(determination of astilbin, rosmarinic acid) was 290 nm; analysis of biomass and total content of effective components. RESULTS Different provenances of Sarcandra glabra biomasses were significantly different, contents of effective components were greatly different, the total content were quite different, the 5 screening high quality provenances had 3 from Fujian Sanming. CONCLUSION The screening of high-quality multi index combined with effective component of total content of biomasses, has great significance to guide scientific planting.