Preparation of Borneol and Brucea Javanica Oil Nanoemulsion and Tumor Suppression Effect on Glioma in Rats

OBJECTIVE To selecte and optimize the prescription of borneol and Brucea Javanica oil nanoemulsion (BBNE), and to prepare the BBNE, then to investigate its tumor suppression effect on glioma in rats. METHODS Establishing the GC analysis methods to determinate BBNE content by pre-column derivatization; applying the techniques of phase inversion to prepare BBNE; selecting and optimizing the prescription of BBNE by pseudo-ternary phase diagrams; using transmission electron microscope and laser granulometer to investigate the appearance shape, determination of particle size distribution and Zeta potential; building glioma tumor model in rats, and investigate BBNE tumor suppression effect by the index of tumor weight and volume. RESULTS The GC analysis method to determinate BBNE content was established successfully, the best prescription of BBNE was oil phase IPM 35%, Brucea Javanica oil (containing 1% borneol) 35%, emulsifier Cremopher RH 18% and Labrasol 12%; the appearance of nanoemulsion was round and spherical, the average particle size was (47.60±0.57)nm, the PDI was (0.22±0.01), the Zeta potential was (?1.06±0.12)mV, and the drug loading capacity was (0.424 1±0.005 6)mg·mL^-1; tumor inhibition experiments showed that the tumor weight and volume of Saline injection group, Brucea Javanica oil injection group, Brucea javanica oil nanoemulsion group and BBNE group were (1.32±0.19), (0.84±0.08), (0.76±0.06), (0.57±0.10)g and (72.2±9.4), (44.2±5.1), (42.3±4.9), (27.9±2.5)mm³, respectively, and the tumor inhibition rates were 36.49%, 42.80%, 56.94%. CONCLUSION The prescription is very well because the BBNE has uniform particle size distribution, higher drug content and better stability, and that BBNE has better tumor suppression effect on glioma in rat show borneol may promote Brucea Javanica oil across the blood-brain barrier to improve tumor suppression effect preliminarily.