Simultaneous Determination of 12 Pesticide Residues in Corydalis Rhizoma by HPLC-MS/MS

OBJECTIVE To establish a method based on LC-MS/MS for the determination of 12 kinds of pesticide residues in Corydalis Rhizoma. METHODS Corydalis Rhizoma samples were extracted with acetonitrile, and purified by N-propylethylenediamine(PSA) column. The prepared samples were analyzed using LC-MS/MS in multiple reaction monitoring(MRM) mode by positive electrospray ionization with a ZORBAX Eclipse plus C18 column as the analytical column. The pesticides were quantified by the internal standard method. RESULTS All the 12 pesticides showed good linearity in their reasonable range(r=0.997 7-0.999 9), the recoveries were 65.5%-117.8%, with the RSDs of 0.4%-4.5%. The limits of detection were 0.1-2.5 μg·kg^-1. CONCLUSION The method established in this study is simple, sensitive and reproductive which can be applied on the analysis of pesticide residues in Corydalis Rhizoma. The quality of Corydalis Rhizoma is basically good for the safety although it contains a trace of several pesticides residues.