Determination of C21-Steroidal-glycoside in Cynanchi Atrati Radix from Different Areas

OBJECTIVE To establish the method for the determination of cynanversicoside A and total C21- steroidal-glycoside in Cynanchi Atrati Radix from different areas. METHODS With the content and absorbance of cynanversicoside A as index, HPLC and UV were used to determine the contents of cynanversicoside A and total C21-steroidal-glycoside in all samples. RESULTS The linear range of cynanversicoside A and steroidal-glycoside was 0.23-2.53 μg and 11.5-115 μg, respectively, with the correlation coefficient of 0.999 7 and 0.999 1; the average recoveries were 99.3% and 99.7%, with RSD of 2.68% and 2.84%. There was great difference of the samples, content of cynanversicoside A of Henan was the highest, lowest in Shandong; the total C21-steroidal-glycoside in sample from Shandong was the highest, lowest in Liaoning. CONCLUSION The method is simple, accurate and reliable for the quality control of Cynanchi Atrati Radix.