Protective Effect of Water Extracts of Sea Cucumber Ovum on Reproductive System of Premature Ovarian Failure Mice

OBJECTIVE To investigate the protective effects of water extracts of sea cucumber ovum on deficiency of the genital system injured by cyclophosphamide. METHODS Female Kunming mice were selected and randomly divided into 6 groups included control group, model group, positive group (estradiol valerate 0.85 mg·kg^-1), and low-, middle-, high-dose sea cucumber ovum group(150, 300, 600 mg·kg^-1) with 10 animals in each group, except the control group injected with normal saline, others were injected with cyclophosphamide(28 mg·kg^-1) daily for successive 5 d to induce reproductive system disorders and premature ovarian failure in mice. Detected the level of estradiol(E2) and progesterone(P) by radioimmunoassay, all mice were compared the weight of organs, such as uterus, ovarian, spleen, and thymus, observed for the form of uterus and ovarian. RESULTS Sea cucumber ovum could increase the weight of organs, such as uterus, ovarian, and thymus gland of mice obviously( P <0.05, P <0.01), significantly improved the level of E2 and P( P <0.05, P <0.01), increased the thickness of endometrial ( P <0.01), improved the form of ovarian obviously. CONCLUSION Sea cucumber ovum can increase the thickness of endometrial, prevent the premature ovarian failure, promote and protect the reproductive system of female mice.